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cdna library lambda zapii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies cdna library lambda zapii vector
    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal <t>cDNA</t> <t>library.</t> The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.
    Cdna Library Lambda Zapii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+library+lambda+zapii+vector/pmc02758064-300-2-10
    Average 90 stars, based on 1 article reviews
    cdna library lambda zapii vector - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation"

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation

    Journal:

    doi: 10.1016/j.neuron.2009.05.024

    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.
    Figure Legend Snippet: (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Techniques Used: Construct, cDNA Library Assay

    Related Articles

    cDNA Library Assay:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..

    Article Title: S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding.
    Article Snippet: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) influences cytotoxicity, translocating to the nucleus during apoptosis.. Here we report a signalling pathway in which nitric oxide (NO) generation that follows apoptotic stimulation elicits S-nitrosylation of GAPDH, which triggers binding to Siah1 (an E3 ubiquitin ligase), nuclear translocation and apoptosis.. S-nitrosylation of GAPDH augments its binding to Siah1, whose nuclear localization signal mediates translocation of GAPDH.

    Plasmid Preparation:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..

    Labeling:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..

    DNA Labeling:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation
    Article Snippet: .. An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim). ..



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    Image Search Results


    (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Journal:

    Article Title: GOSPEL: A novel neuroprotective protein that binds to GAPDH upon S -nitrosylation

    doi: 10.1016/j.neuron.2009.05.024

    Figure Lengend Snippet: (A) Yeast two-hybrid analysis with two GAPDH constructs (amino acids 1-150 or 151-333) and a rat hippocampal cDNA library. The 1-150 amino acid fragment binds with GOSPEL, but not with Siah.

    Article Snippet: An adult rat brain cDNA library in lambda ZAPII vector (Stratagene) was screened using the yeast two-hybrid fragment for a portion of GOSPEL open reading frame, labeled with [ 32 P]dCTP using a random priming DNA labeling kit (Boehringer-Mannheim).

    Techniques: Construct, cDNA Library Assay